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| Registros recuperados : 3 | |
| 1. |  | SEGANFREDO, G. C.; PICCOLI, J. H.; NUNES, E. de O.; MIRANDA, C. R. de; PERIPOLLI, V.; MILLEZI, A. F. Avaliação microbiológica de enterobactérias e salmonella sp. do dejeto suíno recém armazenado em esterqueiras. In: JORNADA DE INICIAÇÃO CIENTÍFICA, 14., 2020, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves: UNC, 2020. p. 54-55.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | SEGANFREDO, G. C.; SEEMANN, L.; NUNES, E. de O.; MIRANDA, C. R. de; PERIPOLLI, V.; MILLEZI, A. F. Viability of enterobacteria in swine manure storage units. Pesquisa Agropecuária Brasileira, v, 57, e02876, 2022. Título em português: Viabilidade de enterobactérias em unidades de armazenamento de dejetos líquidos de suínos.| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| 3. | | SPERANDIO, J.; BASTOS, A. P. A.; FONSECA, F. M. da; BELLAVER, F. A. V.; DAL BELLO, T. C.; KUHNEN, S.; MILLEZI, A. F. Antimicrobial and cytotoxic bioprospection of Thymus Vulgaris and Thymol against Salmonella Enterica Serovar Heidelberg isolated in broiler chicken. Brazilian Archives of Biology and Technology, v. 66, e23210502, 2023.| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| Registros recuperados : 3 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
06/12/2023 |
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Data da última atualização: |
06/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
SPERANDIO, J.; BASTOS, A. P. A.; FONSECA, F. M. da; BELLAVER, F. A. V.; DAL BELLO, T. C.; KUHNEN, S.; MILLEZI, A. F. |
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Afiliação: |
JHONATAN SPERANDIO, Instituto Federal Catarinense/Campus Concórdia; ANA PAULA ALMEIDA BASTOS, CNPSA; FRANCISCO NOE DA FONSECA, GEM; FRANCIANA A VOLPATO BELLAVER, Instituto Federal Catarinense/Campus Concórdia; THAIS CARLA DAL BELLO, Instituto Federal Catarinense/Campus Concórdia; SHIRLEY KUHNEN, Universidade Federal de Santa Catarina; ALESSANDRA FARIAS MILLEZI, Instituto Federal Catarinense/Campus Concórdia. |
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Título: |
Antimicrobial and cytotoxic bioprospection of Thymus Vulgaris and Thymol against Salmonella Enterica Serovar Heidelberg isolated in broiler chicken. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v. 66, e23210502, 2023. |
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DOI: |
https://doi.org/10.1590/1678-4324-2023210502 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Salmonella enterica serovar Heidelberg (SH), often isolated from broiler chicken samples, damages the entire production chain due to its high resistance in the environment. The search for sustainable disinfectant agents has intensified, focusing on the action of essential oils. This work's objective was to evaluate the in vitro, effect of Thymus vulgaris essential oil (TEO) and thymol in SH isolated from broilers, and the cytotoxicity in animal cells. Four different concentrations of TEO (0.1%, 0.2%, 0.4%, and 0.8%, (v/v)) were used against five SH isolates obtained from broilers and ATCC 8326. Thymol was evaluated at the concentrations of 0.023%, 0.047%, 0.071%, and 0.094% (v/v). In vitro antibacterial assays were performed by quantifying viable planktonic cells in the broth microdilution test. The MTT technique was used to assess the cytotoxicity in IEC-6 intestinal cells and NCTC fibroblasts. IC50 was assessed testing the concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% of TEO and thymol in 24 hours. The bacterial activity was observed from the 0.2% concentration of TEO since no colony-forming units were observed. Thymol in the concentration of 0.094% controlled 83.33% of the bacteria. TEO presented an IC50 of 0.14% and 1.22%, while the IC50 for thymol was 0.068% and 0.001% for the IEC-6 and NCTC cells, respectively. On the other hand, TEO showed low cytotoxicity in fibroblasts and a potential action capacity to eliminate SH strains in vitro. |
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Palavras-Chave: |
Antibacterial activity; Atividade antibacteriana; Bioprospecção Antimicrobiana; Serovar Heidelberg; Tomilho. |
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Thesagro: |
Cadeia Produtiva; Frango de Corte; Salmonella Enteritides; Salmonelose. |
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Thesaurus NAL: |
Broiler chickens; Salmonella enterica; Salmonellosis; Sanitizing; Thyme. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159232/1/final9591.pdf
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Marc: |
LEADER 02670naa a2200373 a 4500
001 2159232
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008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/1678-4324-2023210502$2DOI
100 1 $aSPERANDIO, J.
245 $aAntimicrobial and cytotoxic bioprospection of Thymus Vulgaris and Thymol against Salmonella Enterica Serovar Heidelberg isolated in broiler chicken.$h[electronic resource]
260 $c2023
520 $aAbstract: Salmonella enterica serovar Heidelberg (SH), often isolated from broiler chicken samples, damages the entire production chain due to its high resistance in the environment. The search for sustainable disinfectant agents has intensified, focusing on the action of essential oils. This work's objective was to evaluate the in vitro, effect of Thymus vulgaris essential oil (TEO) and thymol in SH isolated from broilers, and the cytotoxicity in animal cells. Four different concentrations of TEO (0.1%, 0.2%, 0.4%, and 0.8%, (v/v)) were used against five SH isolates obtained from broilers and ATCC 8326. Thymol was evaluated at the concentrations of 0.023%, 0.047%, 0.071%, and 0.094% (v/v). In vitro antibacterial assays were performed by quantifying viable planktonic cells in the broth microdilution test. The MTT technique was used to assess the cytotoxicity in IEC-6 intestinal cells and NCTC fibroblasts. IC50 was assessed testing the concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% of TEO and thymol in 24 hours. The bacterial activity was observed from the 0.2% concentration of TEO since no colony-forming units were observed. Thymol in the concentration of 0.094% controlled 83.33% of the bacteria. TEO presented an IC50 of 0.14% and 1.22%, while the IC50 for thymol was 0.068% and 0.001% for the IEC-6 and NCTC cells, respectively. On the other hand, TEO showed low cytotoxicity in fibroblasts and a potential action capacity to eliminate SH strains in vitro.
650 $aBroiler chickens
650 $aSalmonella enterica
650 $aSalmonellosis
650 $aSanitizing
650 $aThyme
650 $aCadeia Produtiva
650 $aFrango de Corte
650 $aSalmonella Enteritides
650 $aSalmonelose
653 $aAntibacterial activity
653 $aAtividade antibacteriana
653 $aBioprospecção Antimicrobiana
653 $aSerovar Heidelberg
653 $aTomilho
700 1 $aBASTOS, A. P. A.
700 1 $aFONSECA, F. M. da
700 1 $aBELLAVER, F. A. V.
700 1 $aDAL BELLO, T. C.
700 1 $aKUHNEN, S.
700 1 $aMILLEZI, A. F.
773 $tBrazilian Archives of Biology and Technology$gv. 66, e23210502, 2023.
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Embrapa Suínos e Aves (CNPSA) |
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